Library-free methylation sequencing with bisulfite padlock probes.

TitleLibrary-free methylation sequencing with bisulfite padlock probes.
Publication TypeJournal Article
Year of Publication2012
AuthorsDiep D, Plongthongkum N, Gore A, Fung H-L, Shoemaker R, Zhang K
JournalNat Methods
Volume9
Issue3
Pagination270-2
Date Published2012 Mar
ISSN1548-7105
KeywordsBase Sequence, DNA, DNA Probes, Gene Library, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, DNA, Sulfites
Abstract

Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.

DOI10.1038/nmeth.1871
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/22306810?dopt=Abstract
PMCPMC3461232
Alternate JournalNat. Methods
PubMed ID22306810