Library-free methylation sequencing with bisulfite padlock probes.
|Title||Library-free methylation sequencing with bisulfite padlock probes.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Diep D, Plongthongkum N, Gore A, Fung H-L, Shoemaker R, Zhang K|
|Date Published||2012 Mar|
|Keywords||Base Sequence, DNA, DNA Probes, Gene Library, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, DNA, Sulfites|
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
|Alternate Journal||Nat. Methods|