Genome-scale metabolic networks.
|Title||Genome-scale metabolic networks.|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Terzer M, Maynard ND, Covert MW, Stelling J|
|Journal||Wiley Interdiscip Rev Syst Biol Med|
|Date Published||2009 Nov-Dec|
|Keywords||Genome, Genome, Bacterial, Genome, Fungal, Metabolic Networks and Pathways, Models, Biological, Reproducibility of Results, Systems Biology|
During the last decade, models have been developed to characterize cellular metabolism at the level of an entire metabolic network. The main concept that underlies whole-network metabolic modeling is the identification and mathematical definition of constraints. Here, we review large-scale metabolic network modeling, in particular, stoichiometric- and constraint-based approaches. Although many such models have been reconstructed, few networks have been extensively validated and tested experimentally, and we focus on these. We describe how metabolic networks can be represented using stoichiometric matrices and well-defined constraints on metabolic fluxes. We then discuss relatively successful approaches, including flux balance analysis (FBA), pathway analysis, and common extensions or modifications to these approaches. Finally, we describe techniques for integrating these approaches with models of other biological processes.
|Alternate Journal||Wiley Interdiscip Rev Syst Biol Med|