Metabolic network analysis integrated with transcript verification for sequenced genomes.

TitleMetabolic network analysis integrated with transcript verification for sequenced genomes.
Publication TypeJournal Article
Year of Publication2009
AuthorsManichaikul A, Ghamsari L, Hom EFY, Lin C, Murray RR, Chang RL, Balaji S, Hao T, Shen Y, Chavali AK, Thiele I, Yang X, Fan C, Mello E, Hill DE, Vidal M, Salehi-Ashtiani K, Papin JA
JournalNat Methods
Volume6
Issue8
Pagination589-92
Date Published2009 Aug
ISSN1548-7105
KeywordsAnimals, Chlamydomonas reinhardtii, Computational Biology, Computer Simulation, Enzymes, Genetic Engineering, Genome, Protozoan, Models, Genetic, Protozoan Proteins, Transcription, Genetic
Abstract

With sequencing of thousands of organisms completed or in progress, there is a growing need to integrate gene prediction with metabolic network analysis. Using Chlamydomonas reinhardtii as a model, we describe a systems-level methodology bridging metabolic network reconstruction with experimental verification of enzyme encoding open reading frames. Our quantitative and predictive metabolic model and its associated cloned open reading frames provide useful resources for metabolic engineering.

DOI10.1038/nmeth.1348
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/19597503?dopt=Abstract
PMCPMC3139173
Alternate TitleNat. Methods
PubMed ID19597503