Dereplication and de novo sequencing of nonribosomal peptides.
|Title||Dereplication and de novo sequencing of nonribosomal peptides.|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Ng J, Bandeira N, Liu W-T, Ghassemian M, Simmons TL, Gerwick WH, Linington R, Dorrestein PC, Pevzner PA|
|Date Published||2009 Aug|
|Keywords||Algorithms, Amino Acid Sequence, Databases, Protein, Mass Spectrometry, Molecular Sequence Data, Peptide Biosynthesis, Nucleic Acid-Independent, Peptide Synthases, Peptides, Cyclic, Sequence Analysis, Protein, Sequence Homology, Amino Acid|
Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but nonidentical NRPs, substantially reducing dereplication efforts.
|Alternate Journal||Nat. Methods|