Dereplication and de novo sequencing of nonribosomal peptides.

TitleDereplication and de novo sequencing of nonribosomal peptides.
Publication TypeJournal Article
Year of Publication2009
AuthorsNg J, Bandeira N, Liu W-T, Ghassemian M, Simmons TL, Gerwick WH, Linington R, Dorrestein PC, Pevzner PA
JournalNat Methods
Volume6
Issue8
Pagination596-9
Date Published2009 Aug
ISSN1548-7105
KeywordsAlgorithms, Amino Acid Sequence, Databases, Protein, Mass Spectrometry, Molecular Sequence Data, Peptide Biosynthesis, Nucleic Acid-Independent, Peptide Synthases, Peptides, Cyclic, Sequence Analysis, Protein, Sequence Homology, Amino Acid
Abstract

Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but nonidentical NRPs, substantially reducing dereplication efforts.

DOI10.1038/nmeth.1350
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/19597502?dopt=Abstract
PMCPMC2754211
Alternate JournalNat. Methods
PubMed ID19597502