Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
|Title||Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||Roguev A, Bandyopadhyay S, Zofall M, Zhang K, Fischer T, Collins SR, Qu H, Shales M, Park H-O, Hayles J, Hoe K-L, Kim D-U, Ideker T, Grewal SI, Weissman JS, Krogan NJ|
|Date Published||2008 Oct 17|
|Keywords||DNA Repair, DNA Replication, Epistasis, Genetic, Gene Expression Regulation, Fungal, Gene Regulatory Networks, Genes, Fungal, Histones, Mutation, RNA Interference, Saccharomyces cerevisiae, Schizosaccharomyces, Schizosaccharomyces pombe Proteins|
An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.