Algorithm for identification of fusion proteins via mass spectrometry.

TitleAlgorithm for identification of fusion proteins via mass spectrometry.
Publication TypeJournal Article
Year of Publication2008
AuthorsNg J, Pevzner PA
JournalJ Proteome Res
Volume7
Issue1
Pagination89-95
Date Published2008 Jan
ISSN1535-3893
KeywordsAlgorithms, Amino Acid Sequence, Databases, Factual, Humans, Mass Spectrometry, Oncogene Proteins, Fusion, Proteomics, Recombinant Fusion Proteins, RNA Splice Sites
Abstract

Identification of fusion proteins has contributed significantly to our understanding of cancer progression, yielding important predictive markers and therapeutic targets. While fusion proteins can be potentially identified by mass spectrometry, all previously found fusion proteins were identified using genomic (rather than mass spectrometry) technologies. This lack of MS/MS applications in studies of fusion proteins is caused by the lack of computational tools that are able to interpret mass spectra from peptides covering unknown fusion breakpoints (fusion peptides). Indeed, the number of potential fusion peptides is so large that the existing MS/MS database search tools become impractical even in the case of small genomes. We explore computational approaches to identifying fusion peptides, propose an algorithm for solving the fusion peptide identification problem, and analyze the performance of this algorithm on simulated data. We further illustrate how this approach can be modified for human exons prediction.

DOI10.1021/pr070214g
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/18173219?dopt=Abstract
Alternate TitleJ. Proteome Res.
PubMed ID18173219