Developmentally regulated activation of a SINE B2 repeat as a domain boundary in organogenesis.

TitleDevelopmentally regulated activation of a SINE B2 repeat as a domain boundary in organogenesis.
Publication TypeJournal Article
Year of Publication2007
AuthorsLunyak VV, Prefontaine GG, Núñez E, Cramer T, Ju B-G, Ohgi KA, Hutt K, Roy R, García-Díaz A, Zhu X, Yung Y, Montoliu L, Glass CK, Rosenfeld MG
JournalScience
Volume317
Issue5835
Pagination248-51
Date Published2007 Jul 13
ISSN1095-9203
KeywordsAnimals, Base Sequence, Chromatin Immunoprecipitation, DNA Polymerase II, DNA Polymerase III, Gene Expression Regulation, Developmental, Growth Hormone, Histones, Insulator Elements, Methylation, Mice, Mice, Transgenic, Molecular Sequence Data, Organogenesis, Pituitary Gland, Short Interspersed Nucleotide Elements, Transcription, Genetic, Transcriptional Activation
Abstract

The temporal and spatial regulation of gene expression in mammalian development is linked to the establishment of functional chromatin domains. Here, we report that tissue-specific transcription of a retrotransposon repeat in the murine growth hormone locus is required for gene activation. This repeat serves as a boundary to block the influence of repressive chromatin modifications. The repeat element is able to generate short, overlapping Pol II-and Pol III-driven transcripts, both of which are necessary and sufficient to enable a restructuring of the regulated locus into nuclear compartments. These data suggest that transcription of interspersed repetitive sequences may represent a developmental strategy for the establishment of functionally distinct domains within the mammalian genome to control gene activation.

DOI10.1126/science.1140871
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/17626886?dopt=Abstract
Alternate TitleScience
PubMed ID17626886