Mass spectrometric detection of tissue proteins in plasma.
|Title||Mass spectrometric detection of tissue proteins in plasma.|
|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Zhang H, Liu AY, Loriaux P, Wollscheid B, Zhou Y, Watts JD, Aebersold R|
|Journal||Mol Cell Proteomics|
|Date Published||2007 Jan|
|Keywords||Antigens, CD, Glycopeptides, Humans, Mass Spectrometry, Neoplasm Proteins|
It has long been thought that blood plasma could serve as a window into the state of one's organs in health and disease because tissue-derived proteins represent a significant fraction of the plasma proteome. Although substantial technical progress has been made toward the goal of comprehensively analyzing the blood plasma proteome, the basic assumption that proteins derived from a variety of tissues could indeed be detectable in plasma using current proteomics technologies has not been rigorously tested. Here we provide evidence that such tissue-derived proteins are both present and detectable in plasma via direct mass spectrometric analysis of captured glycopeptides and thus provide a conceptual basis for plasma protein biomarker discovery and analysis.
|Alternate Journal||Mol. Cell Proteomics|