DyNAVacS: an integrative tool for optimized DNA vaccine design.

TitleDyNAVacS: an integrative tool for optimized DNA vaccine design.
Publication TypeJournal Article
Year of Publication2006
AuthorsHarish N, Gupta R, Agarwal P, Scaria V, Pillai B
JournalNucleic Acids Res
Volume34
IssueWeb Server issue
PaginationW264-6
Date Published2006 Jul 1
ISSN1362-4962
KeywordsCodon, Computational Biology, CpG Islands, DNA Primers, Drug Design, Gene Expression, Internet, Polymerase Chain Reaction, Restriction Mapping, Software, Systems Integration, User-Computer Interface, Vaccines, DNA
Abstract

DNA vaccines have slowly emerged as keystones in preventive immunology due to their versatility in inducing both cell-mediated as well as humoral immune responses. The design of an efficient DNA vaccine, involves choice of a suitable expression vector, ensuring optimal expression by codon optimization, engineering CpG motifs for enhancing immune responses and providing additional sequence signals for efficient translation. DyNAVacS is a web-based tool created for rapid and easy design of DNA vaccines. It follows a step-wise design flow, which guides the user through the various sequential steps in the design of the vaccine. Further, it allows restriction enzyme mapping, design of primers spanning user specified sequences and provides information regarding the vectors currently used for generation of DNA vaccines. The web version uses Apache HTTP server. The interface was written in HTML and utilizes the Common Gateway Interface scripts written in PERL for functionality. DyNAVacS is an integrated tool consisting of user-friendly programs, which require minimal information from the user. The software is available free of cost, as a web based application at URL: http://miracle.igib.res.in/dynavac/.

DOI10.1093/nar/gkl242
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/16845007?dopt=Abstract
PMCPMC1538838
Alternate JournalNucleic Acids Res.
PubMed ID16845007