Parallel adaptive evolution cultures of Escherichia coli lead to convergent growth phenotypes with different gene expression states.

TitleParallel adaptive evolution cultures of Escherichia coli lead to convergent growth phenotypes with different gene expression states.
Publication TypeJournal Article
Year of Publication2005
AuthorsFong SS, Joyce AR, Palsson BØ
JournalGenome Res
Volume15
Issue10
Pagination1365-72
Date Published2005 Oct
ISSN1088-9051
KeywordsBiological Evolution, Escherichia coli, Gene Expression Profiling, Phenotype, RNA, Messenger, Transcription, Genetic
Abstract

Laboratory evolution can be used to address fundamental questions about adaptation to selection pressures and, ultimately, the process of evolution. In this study, we investigated the reproducibility of growth phenotypes and global gene expression states during adaptive evolution. The results from parallel, replicate adaptive evolution experiments of Escherichia coli K-12 MG1655 grown on either lactate or glycerol minimal media showed that (1) growth phenotypes at the endpoint of evolution are convergent and reproducible; (2) endpoints of evolution have different underlying gene expression states; and (3) the evolutionary gene expression response involves a large number of compensatory expression changes and a smaller number of adaptively beneficial expression changes common across evolution strains. Gene expression changes initially showed a large number of differentially expressed genes in response to an environmental change followed by a return of most genes to a baseline expression level, leaving a relatively small set of differentially expressed genes at the endpoint that varied between evolved populations.

DOI10.1101/gr.3832305
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/16204189?dopt=Abstract
PMCPMC1240078
Alternate TitleGenome Res.
PubMed ID16204189