An alternative pluripotent state confers interspecies chimaeric competency.
|Title||An alternative pluripotent state confers interspecies chimaeric competency.|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause MN, Nery JR, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim NYoung, Lajara J, Guillen P, Campistol JM, Esteban CRodriguez, Ross PJ, Saghatelian A, Ren B, Ecker JR, Izpisua Belmonte J C|
|Date Published||2015 May 21|
|Keywords||Animals, Cell Culture Techniques, Cell Line, Chimera, Embryonic Stem Cells, Female, Germ Layers, Humans, Induced Pluripotent Stem Cells, Male, Mice, Pan troglodytes, Pluripotent Stem Cells, Regenerative Medicine, Species Specificity|
Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture parameters, a stem-cell type with unique spatial characteristics and distinct molecular and functional features, designated as region-selective pluripotent stem cells (rsPSCs), can be efficiently obtained from mouse embryos and primate pluripotent stem cells, including humans. The ease of culturing and editing the genome of human rsPSCs offers advantages for regenerative medicine applications. The unique ability of human rsPSCs to generate post-implantation interspecies chimaeric embryos may facilitate our understanding of early human development and evolution.
|Grant List||/ / Howard Hughes Medical Institute / United States|