Using ultradeep pyrosequencing to study HIV-1 coreceptor usage in primary and dual infection.

TitleUsing ultradeep pyrosequencing to study HIV-1 coreceptor usage in primary and dual infection.
Publication TypeJournal Article
Year of Publication2013
AuthorsWagner GA, Pacold ME, Vigil E, Caballero G, Morris SR, Kosakovsky Pond SL, Little SJ, Richman DD, Gianella S, Smith DM
JournalJ Infect Dis
Volume208
Issue2
Pagination271-4
Date Published2013 Jul 15
ISSN1537-6613
KeywordsAdult, HIV Infections, HIV-1, Humans, Male, Receptors, CXCR4, Sequence Analysis, DNA
Abstract

HIV-1 dual infection (DI) and CXCR4 (X4) coreceptor usage are associated with accelerated disease progression but frequency and dynamics of coreceptor usage during DI is unknown. Ultradeep sequencing was used to interrogate for DI and infer coreceptor usage in longitudinal blood samples of 102 subjects. At baseline, X4 usage was high (23 subjects harbored X4 variants) and was not associated with infection duration or DI. Coreceptor usage changed over time in 12 of 47 participants, and X4 usage emerged in 4 of 41 monoinfections vs 2 of 5 superinfections (P = .12), suggesting a weak statistical trend toward occurrence of superinfection and acquiring X4 usage.

DOI10.1093/infdis/jit168
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/23599311?dopt=Abstract
PMCPMC3685231
Alternate JournalJ. Infect. Dis.
PubMed ID23599311