Using ultradeep pyrosequencing to study HIV-1 coreceptor usage in primary and dual infection.
|Title||Using ultradeep pyrosequencing to study HIV-1 coreceptor usage in primary and dual infection.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Wagner GA, Pacold ME, Vigil E, Caballero G, Morris SR, Kosakovsky Pond SL, Little SJ, Richman DD, Gianella S, Smith DM|
|Journal||J Infect Dis|
|Date Published||2013 Jul 15|
|Keywords||Adult, HIV Infections, HIV-1, Humans, Male, Receptors, CXCR4, Sequence Analysis, DNA|
HIV-1 dual infection (DI) and CXCR4 (X4) coreceptor usage are associated with accelerated disease progression but frequency and dynamics of coreceptor usage during DI is unknown. Ultradeep sequencing was used to interrogate for DI and infer coreceptor usage in longitudinal blood samples of 102 subjects. At baseline, X4 usage was high (23 subjects harbored X4 variants) and was not associated with infection duration or DI. Coreceptor usage changed over time in 12 of 47 participants, and X4 usage emerged in 4 of 41 monoinfections vs 2 of 5 superinfections (P = .12), suggesting a weak statistical trend toward occurrence of superinfection and acquiring X4 usage.
|Alternate Journal||J. Infect. Dis.|